E8406

抗EDEM1 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: 抗EDEM抗体, 抗ER分解促進因子抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 65 kDa (nonglycosylated human and mouse EDEM1); antigen 75 kDa
• 化学種の反応性: human, mouse
• テクニック: immunoprecipitation (IP): 0.5-1.0 μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.; western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.
• UniProtアクセッション番号: P20472
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EDEM1(9695) ; mouse ... Pvalb(19293)

詳細

詳細

Anti-EDEM1 recognizes glycoslylated human and mouse EDEM1 with molecular weight 75 kDa and nonglycosylated human and mouse EDEM1 with a molecular
weight 65 kDa.

ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1) is an enzyme encoded by the EDEM1 gene in humans. In mammalian cells, EDEM1 is localized to the endoplasmic reticulum (ER), mainly as a soluble glycoprotein.

免疫原

ヒトEDEM1のアミノ酸629~646に相当する合成ペプチドのN末端システイン残基を介したKLH結合体。この配列はマウスEDEM1内の配列と同一です。

アプリケーション

Anti-EDEM1 antibody has been used in immunoblotting.

Anti-EDEM1 antibody produced in rabbit is suitable for the following applications:

• Immunoprecipitation at a concentration of 0.5-1.0μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.
• Western blotting at a concentration of 0.5-1.0μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.
• Immunofluorescence Microscopy.
• Immunoblotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

生物化学的／生理学的作用

ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1), a putative mannose binding lectin, targets misfolded glycoproteins for degradation in an N-glycan dependent manner. It interacts with the C-terminus of calnexin and lacks mannosidase activity.

ER degradation-enhancing alpha-mannosidase-like 1 is an enzyme encoded by the EDEM1 gene in humans. It is a chaperone for rod opsin and its expression can be used to promote correct folding enhancing degradation of mutant proteins in the ER to combat protein-misfolding disease. The endogenous EDEM1 in cells that are not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophagy. EDEM may maintain the retrotranslocation competence of NHK by inhibiting aggregation. It is also considered responsible for directing aberrant proteins for ERAD (ER-associated protein degradation).

物理的形状

15 mMアジ化ナトリウム含有のpH 7.4の0.01 Mリン酸緩衝食塩水溶液.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)