SK-N-DZ

94092305, human nerve (bone marrow metastasis), Neuronal

• UNSPSCコード: 41106514

特性

• product name: SK-N-DZ, 94092305
• 由来生物: human nerve (bone marrow metastasis)
• 成長モード: Adherent
• 核型: 44XX, monosomic for 10, 11, 13, 14, 19. Both copies of chromosome 2 missing in each cell.
• 形態: Neuronal
• 製品: p53, moderate MDR-1 expression
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: metastasis
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human neuroblastoma

細胞株の説明

Spontaneously transformed cells from a female patient with neuroblastoma. The tumour was derived from a bone marrow metastasis and is tumourigenic in nude mice. Used in the study of MIBG uptake in the diagnosis and treatment of neuroblastoma. MIBG is a derivative of the antihypertensive agent guanethidine and is used as a radiopharmaceutical. SK-N-DZ is characterised by low saturable kinetics and predominant linear pattern of uptake of MIBG, with a poor ability to maintain high intracellular levels of the drug.

アプリケーション

Tumour studies. MRD-1 resistance studies. Extremely sensitive to cis-retinoic acid.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,11
D5S818: 12
D7S820: 12,13
THO1: 6,9
TPOX: 8
vWA: 16,18

培地

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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