The glioma cell line A10-85 is a subclone of A10 cells which originally emerged from the parent line IA2 by reducing the serum concentration from 15% to 10%. This parent line IA2 was derived from a rat brain glioma induced transplacentally by ethylnitroso urea (inbred BDIxrats). A10-85 cells form processes in response to cAMP. GAPDH can be induced by hydrocortisone succinate and good growth was obtained in semi-solid medium. Injection of cells into syngeneric animals resulted in the formation of malignant astrocytic tumours after short latent periods.
Split sub-confluent cultures (70-80%) 1:50 to 1:100 seeding at 5x10,000 to 1x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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