GH1

89072610, rat brain, Epithelial

• UNSPSCコード: 41106514

特性

• product name: GH1, 89072610
• 由来生物: rat brain
• 成長モード: Semi-adherent
• 核型: Hypodiploid, modal no. 40.
• 形態: Epithelial
• 製品: Rat growth hormone, lactogenic hormones.
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat Wistar-Furth pituitary tumour

細胞株の説明

Derived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1μg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.

アプリケーション

Hormone studies

培地

Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells b

その他情報

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