コンテンツへスキップ
Merck

T1323

Sigma-Aldrich

モノクロナール抗ε-チューブリン マウス宿主抗体

clone TUB-11, purified immunoglobulin, buffered aqueous solution

ログイン組織・契約価格を表示する


About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

結合体

unconjugated

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

TUB-11, monoclonal

形状

buffered aqueous solution

分子量

antigen 55 kDa

化学種の反応性

frog, mouse, chicken, human

テクニック

immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using whole extract of cultured chicken fibroblasts

アイソタイプ

IgG2a

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... TUBE1(51175)
mouse ... Tube1(71924)

詳細

Monoclonal Anti-ε-Tubulin (mouse IgG2a isotype) is derived from the TUB-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide.
Tubulin epsilon 1 (ε-tubulin) is a member of tubulin superfamily. ε-tubulin is localized to the centrosome in cell-cycle-dependent manner. It is encoded by the gene mapped to human chromosome 6q21.

特異性

Monoclonal Anti-ε-Tubulin recognizes human, mouse, frog, and chicken ε-tubulin (55 kDa).

免疫原

ヒトεチュ-ブリンのアミノ酸352~366に相当する合成ペプチドのKLH結合体。

アプリケーション

Monoclonal Anti-ε-Tubulin antibody produced in mouse may be used in:
  • immunoblotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunofluorescence

生物化学的/生理学的作用

Tubulin ε 1 (ε-Tubulin) plays a vital role in microtubule anchoring and centriole maturation.

物理的形状

0.01M PBS溶液(pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

保管および安定性

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

適切な製品が見つかりませんか。  

製品選択ツール.をお試しください


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Centrosome composition and microtubule anchoring mechanisms
Bornens M.
Current Opinion in Cell Biology, 14, 25-34 (2002)
ε-Tubulin Is an Essential Component of the Centriole
Dutcher SK
Molecular Biology of the Cell, 13, 3859?3869-3859?3869 (2002)
P G McKean et al.
Journal of cell science, 114(Pt 15), 2723-2733 (2001-10-31)
Although most eukaryotic cells can express multiple isotypes of alphabeta-tubulin, the significance of this diversity has not always been apparent. Recent data indicate that particular alphabeta-tubulin isotypes, both genome encoded and those derived by post-translational modification, can directly influence microtubule
Paul Chang et al.
Nature cell biology, 5(1), 71-76 (2003-01-03)
Centrosomes nucleate microtubules and serve as poles of the mitotic spindle. Centrioles are a core component of centrosomes and duplicate once per cell cycle. We previously identified epsilon-tubulin as a new member of the tubulin superfamily that localizes asymmetrically to
Nafees Ahmad et al.
The International journal of developmental biology, 57(9-10), 741-751 (2013-12-07)
Pitx3 is a bicoid-related homeodomain transcription factor critical for the development of the ocular lens, mesencephalic dopaminergic neurons and skeletal muscle. In humans, mutations in PITX3 are responsible for cataracts and anterior segment abnormalities of varying degree; polymorphisms are associated

資料

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

真核生物の細胞骨格の微小管は、αチューブリンとβチューブリンのヘテロダイマーで構成されています。αおよびβチューブリンに加えて、いくつかの他のチューブリンが同定されており、現在、明確なチューブリンクラスの数は7種類になっています。

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)