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リコンビナント
expressed in E. coli (AP401 (k))
形状
solution
使用法
sufficient for 100 reactions
sufficient for 20 reactions
包装
pkg of 1,000 U (11785826001 [20 μl])
pkg of 5,000 U (11785834001 [100 μl])
メーカー／製品名
Roche
パラメーター
42-43 °C optimum reaction temp.
テクニック
RT-PCR: suitable
RT-qPCR: suitable
入力
purified RNA
検出方法
probe-based
保管温度
−20°C
詳細
Expand™ Reverse Transcriptase is a RNA directed DNA polymerase optimized for amplification of cDNA fragments up to 13.5 kb in a two-step RT-PCR assay. The enzyme is a genetically engineered version of the Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV-RT). A point mutation within the RNase H sequence reduces RNase H activity to undetectable levels. Detectable improvements are obtained with respect to higher amounts of full-length cDNA transcripts and longer transcripts compared to using the native M-MuLV-RT.
特異性
Heat inactivation: 95 °C for 2 min
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
アプリケーション
Expand™ Reverse Transcriptase has been used in real-time PCR analysis for conversion of isolated RNA into cDNA. It has also been used in reverse transcription (RT). Expand™ Reverse Transcriptase may also be used for analyzing the sequence of the cDNA and comparing it to the genomic sequence. Important information can be obtained on intervening sequences (introns) and genomic recombinations.
特徴および利点
- Obtain higher amounts of full-length cDNA transcripts and longer transcripts up to 13.5kb
- No RNase H activity
- Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
- Ideal for two-Step RT-PCR assay
- Purification of cDNA before PCR reaction is not necessary
包装
1 set containing 3 components
単位の定義
One unit is the enzyme activity that incorporates 1.0 nmol TMP into acid insoluble products in 10 min at +37 °C with poly(A)+ × (dT)15 as substrate.
Volume Activity: 50 U/μl
Volume Activity: 50 U/μl
その他情報
For life science research only. Not for use in diagnostic procedures.
法的情報
Expand is a trademark of Roche
キットの構成要素のみ
製品番号
詳細
- Expand Reverse Transcriptase 50 U/μl
- Expand Reverse Transcriptase Buffer 5x concentrated
- DTT Stock Solution 100 mM
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Eye Irrit. 2
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(℃)
does not flash
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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関連コンテンツ
RT-qPCRまたは定量的逆転写PCR法は、逆転写と定量的PCRまたはリアルタイムPCRの働きを組み合わせて特定のターゲットを増幅し検出するものです。RT-qPCRには、遺伝子発現レベルの定量化、RNA干渉（RNAi）の検証、ウイルスなどの病原体の検出など、さまざまな用途があります。
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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