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由来生物
rat
品質水準
結合体
unconjugated
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
SUSI63B, monoclonal
分子量
calculated mol wt 144.44 kDa
observed mol wt ~145 kDa
精製方法
using protein G
化学種の反応性
human, mouse
包装
antibody small pack of 100 μL
テクニック
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
アイソタイプ
IgG2aκ
エピトープ配列
N-terminal
タンパク質IDアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... Pnpla3(116939)
詳細
Cohesin subunit SA-1 (UniProt: Q9D3E6; also known as SCC3 homolog 1, Stromal antigen 1) is encoded by the Stag1 (also known as Sa1) gene (Gene ID: 20842) in murine species. Cohesin are known to mediate sister chromatid cohesion and 3D genome folding. They hold the sister chromatids together to ensure correct DNA repair by homologous recombination and proper chromosome segregation during cell division. They are essential for cell proliferation. The cohesin complex forms a large proteinaceous ring within which sister chromatids can be trapped and at anaphase stage. The complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. This complex may also play a role in spindle pole assembly during mitosis. Two variant cohesin complexes containing Structural Maintenance of Chromosomes (SMC) 1, SMC3, RAD21 and either STAG/SA1 or STAG2/SA2 are present in all cell types. Before prophase, STAG1/SA1 appears to be scattered along chromosome arms. However, during prophase, most of cohesin complexes dissociate from chromatin. This dissociation is probably due to its phosphorylation by PLK1. At anaphase, the RAD21 subunit is cleaved that leads to the dissociation of the complex from chromosomes, allowing their separation. (Ref.: De Koninck, M., et al. (2020). Cell Rep.32(6); 108014; Kojic, A., et al. (2018). Nat. Struct. Mol. Biol. 25(6); 496-504).
特異性
Clone SUSI63B is a rat monoclonal antibody that detects STAG1/SA1. It targets an epitope within the N-terminal region.
免疫原
MBP-tagged recombinant fragment corresponding to the first 225 amino acids from N-terminal region of mouse Cohesin subunit SA-1 (STAG1).
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected STAG1/SA1 in MCF-7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected STAG1/SA1 in HeLa and RAW264.7 cell lysates.
Flow Cytometry Analysis: A representative lot detected STAG1/SA1 in Flow Cytometry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Immunofluorescence Analysis: A representative lot detected STAG1/SA1 in Immunofluorescence applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Western Blotting Analysis: A representative lot detected STAG1/SA1 in Western Blotting applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014; Kojic, A., et al. (2018). Nat Struct Mol Biol. 25(6): 496-504; Cuadrado, A., et al. (2019). Cell Rep. 27(12): 3500-3510.e4).
Immunohistochemistry Applications: A representative lot detected Stag1 in Immunohistochemistry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected STAG1/SA1 in MCF-7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected STAG1/SA1 in HeLa and RAW264.7 cell lysates.
Flow Cytometry Analysis: A representative lot detected STAG1/SA1 in Flow Cytometry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Immunofluorescence Analysis: A representative lot detected STAG1/SA1 in Immunofluorescence applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Western Blotting Analysis: A representative lot detected STAG1/SA1 in Western Blotting applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014; Kojic, A., et al. (2018). Nat Struct Mol Biol. 25(6): 496-504; Cuadrado, A., et al. (2019). Cell Rep. 27(12): 3500-3510.e4).
Immunohistochemistry Applications: A representative lot detected Stag1 in Immunohistochemistry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-STAG1/SA1, clone SUSI63B, Cat. No. MABE2037, is a rat monoclonal antibody that detects STAG1/SA1 and is tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
物理的形状
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
保管および安定性
Recommended storage: +2°C to +8°C.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE2037-25UL:
MABE2037-100UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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