詳細
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Sphingosine 1-phosphate (S1P) is a biologically active lysophospholipid that transmits signals through a family of five G-protein-coupled receptors to regulate cell proliferation, migration, cytoskeletal organization, and differentiation (Spiegel and Milstien , 2003). S1P4 receptor was reported to couple to multiple subsets of heterotrimeric G proteins (including Gi/o, and G12/13, and possibly Gs). Unlike other S1P receptors, S1P4 expression is restricted in human and mouse to lymph node, spleen, lung, and thymus (Ishii et al. 2001). This expression pattern suggests potential roles of S1P4 in the immune system. Millipore′s cloned human S1P4-expressing cell line is made in the Chem-5 host, which supports high levels of recombinant S1P4 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at S1P4.
Sphingosine 1-phosphate (S1P) is a biologically active lysophospholipid that transmits signals through a family of five G-protein-coupled receptors to regulate cell proliferation, migration, cytoskeletal organization, and differentiation (Spiegel and Milstien , 2003). S1P4 receptor was reported to couple to multiple subsets of heterotrimeric G proteins (including Gi/o, and G12/13, and possibly Gs). Unlike other S1P receptors, S1P4 expression is restricted in human and mouse to lymph node, spleen, lung, and thymus (Ishii et al. 2001). This expression pattern suggests potential roles of S1P4 in the immune system. Millipore′s cloned human S1P4-expressing cell line is made in the Chem-5 host, which supports high levels of recombinant S1P4 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at S1P4.
細胞株の説明
- GPCR Cell Lines
- Host cells: Chem-5
アプリケーション
Human S1P4 / EDG6 GPCR frozen cells for Calcium Flux FLIPR Assays.
生物化学的／生理学的作用
- GPCR Class: A
- Protein Target: S1P4 / EDG6
- Target Sub-Family: Lysophospholipid
構成
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
免責事項
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
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名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
HTS192RTA:
試験成績書（COA）
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