ABC61-M

Anti-NIPP-1 Antibody

from rabbit, purified by affinity chromatography

• 別名: Nuclear inhibitor of protein phosphatase 1, NIPP-1, Protein phosphatase 1 regulatory inhibitor subunit 8, ARD-1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 300
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human, mouse
• テクニック: immunocytochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_002704
• UniProtアクセッション番号: Q12972
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPP1R8(5511)

詳細

詳細

Also known as ARD1 (Activator of RNA decay), NIPP-1 (nuclear inhibitor of protein phosphatase 1) is a ubiquitously expressed multifunctional nuclear scaffold protein of 39 kDa, which plays an important role in integrating transcription, RNA processing, and cell cycle progression. NIPP-1 serves as a signaling hub in the nucleus. Additionally, NIPP-1 contains at least two binding sites for PP1 and is complexed to about one-third of the nuclear pool of PP1. Normally, the NIPP-1 - PP1 complex is inactive, but can be activated following phosphorylation of NIPP-1 with PKA, CFK2, or Src family kinases. NIPP-1 also interacts directly with nucleic acids and components of polycomb repressive complexe 2 (PRC2) and functions as a PRC2-dependent transcriptional repressor.

特異性

This antibody should recognize the alpha and beta isoforms of NIPP-1.

免疫原

GST-tagged recombinant protein corresponding to human NIPP-1.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
エピジェネティクス

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NIPP-1 in Raji cells.

This NIPP-1 antibody is validated for use in WB & ICC for the detection of the NIPP-1 protein.

品質

Evaluated by Western Blot Analysis in Raji nuclear extract.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected NIPP-1 in 10 µg of Raji nuclear extract.

ターゲットの説明

~42 kDa observed. Three isoforms at 39 kDa (alpha isoform), 23 kDa (beta isoform), and 13 kDa (gamma isoform) are known to exist. An uncharacterized band may be observed at ~62 kDa in some nuclear extracts.

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Raji nuclear extract

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids