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由来生物
rabbit
品質水準
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
rat
メーカー／製品名
Chemicon®
テクニック
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... KCND2(3751)
詳細
This Anti-Potassium Channel Kv4.2 Antibody is validated for use in WB for the detection of Potassium Channel Kv4.2.
特異性
Recognizes Kv4.2 (Shal1, RK5, Kcnd2). The epitope specific for Kv4.2 is not present in any other known proteins.
SPECIES REACTIVITIES: Rat. Reactivity with other species has not yet been tested. It is expected to work on mouse and human because the immunogen sequence is highly homologous (15/16 residues identical).
SPECIES REACTIVITIES: Rat. Reactivity with other species has not yet been tested. It is expected to work on mouse and human because the immunogen sequence is highly homologous (15/16 residues identical).
免疫原
Highly purified peptide corresponding to residues 454-469 of rat Kv4.2 (Accession Q63881).
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
イオンチャネル及びトランスポーター
イオンチャネル及びトランスポーター
Anti-Potassium Channel Kv4.2 Antibody is an antibody against Potassium Channel Kv4.2 for use in WB.
Western blotting: 1:200 using ECL on rat brain membranes.
Immunohistochemistry on rat brain sections.
Immunoprecipitation Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on rat brain sections.
Immunoprecipitation Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
ターゲットの説明
70 kDa
物理的形状
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, and 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
保管および安定性
Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
アナリシスノート
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
毒物
Jan Code
AB5360-50UL:
AB5360-200UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Wei Zhou et al.
Neuron, 41(4), 573-586 (2004-02-26)
Four Kv channel-interacting proteins (KChIP1 through KChIP4) interact directly with the N-terminal domain of three Shal-type voltage-gated potassium channels (Kv4.1, Kv4.2, and Kv4.3) to modulate cell surface expression and function of Kv4 channels. Here we report a 2.0 Angstrom crystal
Henry H Jerng et al.
The Journal of physiology, 568(Pt 3), 767-788 (2005-08-27)
Kv4 pore-forming subunits are the principal constituents of the voltage-gated K+ channel underlying somatodendritic subthreshold A-type currents (I(SA)) in neurones. Two structurally distinct types of Kv4 channel modulators, Kv channel-interacting proteins (KChIPs) and dipeptidyl-peptidase-like proteins (DPLs: DPP6 or DPPX, DPP10
Daniel I Levy et al.
The Journal of physiology, 588(Pt 14), 2657-2668 (2010-05-26)
MiRP3, the single-span membrane protein encoded by KCNE4, is localized by immunofluorescence microscopy to the transverse tubules of murine cardiac myocytes. MiRP3 is found to co-localize with Kv4.2 subunits that contribute to cardiac transient outward potassium currents (I(to)). Whole-cell, voltage-clamp
Jochen Rose et al.
American journal of physiology. Heart and circulatory physiology, 288(5), H2077-H2087 (2005-01-08)
Action potential (AP) prolongation is a hallmark of failing myocardium. Functional downregulation of K currents is a prominent feature of cells isolated from failing ventricles. The detailed changes in K current expression differ depending on the species, the region of
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