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由来生物
rabbit
品質水準
抗体製品の状態
serum
クローン
polyclonal
化学種の反応性
human, rat
メーカー/製品名
Chemicon®
テクニック
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
遺伝子情報
human ... CALB2(794)
詳細
Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.
特異性
Reactivity with other species has not been determined
Specific for calretinin. Recognizes both the calcium-bound and calcium-unbound conformations of calretinin by western blots.
免疫原
Recombinant rat calretinin.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経シグナリング
神経シグナリング
This Anti-Calretinin Antibody is validated for use in IH, IH(P), WB for the detection of Calretinin.
Western blot:
1:1,000-1:2,000 of a previous lot was used.
Immunohistochemistry:
1:1,000-1:10,000
Antibody works in paraffin embedded tissues.
Optimal working dilutions must be determined by the end user.
1:1,000-1:2,000 of a previous lot was used.
Immunohistochemistry:
1:1,000-1:10,000
Antibody works in paraffin embedded tissues.
Optimal working dilutions must be determined by the end user.
品質
Immunohistochemistry(paraffin) Analysis:
Calretinin (cat. # AB5054) staining on Human Cerebellum. Tissue pretreated with Citrate, pH 6.0 plus. Antibody was diluted to 1:200 (5.0 µg/mL), using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as a nuclear and cytoplasmic punctate staining on neurons (brown).
Optimal Staining With Citrate Buffer Epitope Retrieval: Human Cerebellum.
Calretinin (cat. # AB5054) staining on Human Cerebellum. Tissue pretreated with Citrate, pH 6.0 plus. Antibody was diluted to 1:200 (5.0 µg/mL), using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as a nuclear and cytoplasmic punctate staining on neurons (brown).
Optimal Staining With Citrate Buffer Epitope Retrieval: Human Cerebellum.
関連事項
Replaces: AB149
物理的形状
Unpurified
Rabbit polyclonal serum contains no preservative.
保管および安定性
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
Rat brain cortex, Mesothelioma tissue, Human cerebellum.
Rat brain cortex, Mesothelioma tissue, Human cerebellum.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB5054:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Calcium-binding Protein Calretinin Immunoreactivity in the Dog Superior Colliculus.
Lee, Jea-Young, et al.
Acta Histochemica Et Cytochemica, 39, 125-138 (2006)
Repeated phencyclidine in monkeys results in loss of parvalbumin-containing axo-axonic projections in the prefrontal cortex.
Bret A Morrow, John D Elsworth, Robert H Roth
Psychopharmacology (Psychopharmacologia) null
Immunohistochemical and histochemical characteristics of the olfactory system of the guppy, Poecilia reticulata (Teleostei, Poecilidae).
Bettini S, Lazzari M, Ciani F, Franceschini V
Anatomical Record (Hoboken, N.J. : 2007) null
Allison Walter et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(9), 3360-3369 (2010-05-06)
This study evaluated the in vivo antitumor activity of grape-derived polyphenols. BALB/c mice were subcutaneously implanted with C26 colon carcinoma cells, and 2 d later they received either solvent or red wine polyphenols (RWPs) (100 mg/kg/d, human equivalent dose approximately
Hippocampal and visuospatial learning defects in mice with a deletion of frizzled 9, a gene in the Williams syndrome deletion interval.
Zhao, C; Aviles, C; Abel, RA; Almli, CR; McQuillen, P; Pleasure, SJ
Development null
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