16-101

Anti-Phosphotyrosine Antibody, clone 4G10^®, agarose conjugate

clone 4G10®, Upstate^®, from mouse

• 別名: 4G10 Phosphotyrosine Antibody, Clone 4G10 Anti-Phosphotyrosine, Phosphotyrosine Detection Antibody
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 4G10®, monoclonal
• 化学種の反応性: vertebrates
• メーカー／製品名: Upstate^®
• テクニック: immunoprecipitation (IP): suitable
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PID1(55022)

詳細

詳細

Anti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate

Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

特異性

Recognizes tyrosine-phosphorylated proteins from all species.

免疫原

Phosphotyramine-KLH.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
一般的な翻訳後修飾

Anti-Phosphotyrosine Antibody, clone 4G10, agarose conjugate is an antibody against Phosphotyrosine for use in IP.

This product is useful for immunoprecipitation and immunoaffinity chromatography methods.

Suitable for immunoprecipitation and affinity purification.

特徴および利点

Format: Gel Immobilized

品質

Evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.

Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.

ターゲットの説明

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

物理的形状

Protein A purified

1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% Kathon as a preservative, for a total volume of 2 mL. Liquid suspension.

保管および安定性

Stable for 1 year at 2-8ºC from date of receipt.

アナリシスノート

Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2