リコンビナント
expressed in baculovirus infected Sf21 cells
品質水準
詳細
protein target PI3K-C2α
精製方法
immobilized metal affinity chromatography (IMAC)
テクニック
activity assay: suitable
UniProtアクセッション番号
遺伝子情報
human ... PIK3CG(5294)
詳細
N-terminal 6His-tagged recombinant human PI3K-C2α amino acids 299–end.
包装
Also available in 250μg size as catalog # 14-906M - Call for pricing and availability.
品質
Routinely evaluated in the PIProfiler.
物性
161kDa
物理的形状
10 μg of enzyme in 10.6 μl of 50 mM Tris/HCl, pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol, 20 mM β-glycerophosphate, 0.25 mM Na3VO4, 10 mM NaF. Frozen solution.
保管および安定性
6 months at -70°C
アナリシスノート
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
その他情報
FUNCTION: Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. May play a role in clathrin-coated endocytic vesicle formation and EGF signaling cascade. May be involved in mitosis and UV-induced damage response. May be a downstream effector in insulin signaling cascade.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate.
COFACTOR: Calcium or magnesium. Manganese cannot be used.
ENZYME REGULATION: Activated by insulin. Only slightly inhibited by wortmannin and LY294002. Activated by clathrin.
Subunit structure: Part of a complex with ERBB2 and EGFR. Interacts with clathrin trimers.
SUBCELLULAR LOCATION: Cell membrane. Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle. Nucleus. Cytoplasm. Associated with RNA-containing structures.
TISSUE SPECIFICITY: Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney.
SIMILARITY: Belongs to the PI3/PI4-kinase family. Contains 1 C2 domain. Contains 1 PI3K/PI4K domain. Contains 1 PX (phox homology) domain.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate.
COFACTOR: Calcium or magnesium. Manganese cannot be used.
ENZYME REGULATION: Activated by insulin. Only slightly inhibited by wortmannin and LY294002. Activated by clathrin.
Subunit structure: Part of a complex with ERBB2 and EGFR. Interacts with clathrin trimers.
SUBCELLULAR LOCATION: Cell membrane. Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle. Nucleus. Cytoplasm. Associated with RNA-containing structures.
TISSUE SPECIFICITY: Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney.
SIMILARITY: Belongs to the PI3/PI4-kinase family. Contains 1 C2 domain. Contains 1 PI3K/PI4K domain. Contains 1 PX (phox homology) domain.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Skin Sens. 1
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
劇物
Jan Code
14-906:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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