14-742-M

FGFR2 (N549H) Protein, active, 10 µg

Active, N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770. For use in Kinase Assays.

• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

特性

• 由来生物: human
• 品質水準: 300
• リコンビナント: expressed in baculovirus infected Sf21 cells
• 比活性: 6250 U/mg
• 分子量: M_w 38.1 kDa
• メーカー／製品名: Upstate^®
• テクニック: activity assay: suitable (kinase)
• NCBIアクセッション番号: NM_000141.3; NM_022970.2
• UniProtアクセッション番号: P21802
• 遺伝子情報: human ... FGFR2(2263)

詳細

詳細

N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770.

Product Source: recombinant, human FGFR2 (N549H), amino acids 456-770, expressed by baculovirus in Sf21 insect cells.

アプリケーション

Research Category
代謝

Research Sub Category
心血管疾患

代謝障害

生物化学的／生理学的作用

Protein Target: FGFR2

Target Sub-Family: TK

品質

Routinely evaluated by phosphorylation of 500 μM GGEEEEYFELVKKKK.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of active GFGR2 (N549H).

単位の定義

Specific Activity: where one unit of FGFR2 (N549H) activity is defined as 1nmol phosphate incorporated into 500μM GGEEEEYFELVKKKK per minute at 30°C with a final ATP concentration of 100µM.

物理的形状

Ni²⁺/NTA-agarose

10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

保管および安定性

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable