07-415-I
Anti-Sam 68 Antibody
serum, from rabbit
別名:
KH domain-containing, RNA-binding, signal transduction-associated protein 1, GAP-associated tyrosine phosphoprotein p62, Src-associated in mitosis 68 kDa protein, Sam68, p21 Ras GTPase-activating protein-associated p62, p68
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すべての画像(3)
About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, mouse
化学種の反応性（ホモロジーによる予測）
rat (based on 100% sequence homology)
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... KHDRBS1(10657)
mouse ... Khdrbs1(20218)
rat ... Khdrbs1(117268)
詳細
KH domain-containing, RNA-binding, signal transduction-associated protein 1 (UniProt Q60749; also known as GAP-associated tyrosine phosphoprotein p62, Khdrbs1,p68, p21 Ras GTPase-activating protein-associated p62, Sam68, Src-associated in mitosis 68 kDa protein) is encoded by the Khdrbs1 gene (Gene ID 20218) in murine species. Sam68 is a member of the “signal transduction and activation of RNA” (STAR) family of KH domain-containing RNA-binding proteins that mediates pre-mRNA splicing, mRNA export, and protein translation. It binds to UAAA, UUUA, and UUUUUU motifs with high affinity. RNA immunoprecipitation (RIP) techniques using lysates from UV cross-linked NIH 3T3 cells have identified 23 Sam68 complex-associated mRNA species, including Thrap1, bri3bp, Myl6, Copeb/KLF6/Zf9, Hmgn1, Smc6, and biglycan. In addition, Sam68 is found to be a key regulator of Nrxn1 alternative splicing in the central nervous system in a neuronal activity-dependent manner. Sam68 is phosphorylated on three tyrosine residues (pTyr435, pTyr440, and pTyr443) within the C-terminal nuclear localization signal (NLS) region by the breast tumor kinase (BRK) following EGF stimulation of breast tumor cells with pTyr440 being a principal modulator of Sam68 nuclear localization. Studies comparing wild-type and Sam68-/- knockout mice revealed a positive regulatory role of Sam68 toward adipocyte differentiation, as well as a negative impact of Sam68 against osteoblast differentiation among aged mice.
特異性
This antiserum (also known as AD-1 or AD1) recognizes Sam68, but not two other GSG (GRP33, Sam68, GLD-1) proteins, the Sam68-like mammalian proteins SLM-1 and SLM-2.
免疫原
Epitope: aa 331-348
KLH-conjugated peptide corresponding to amino acid residues 331-348 of human Sam 68
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Research Sub Category
神経シグナリング
神経シグナリング
This Anti-Sam 68 Antibody is validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation for the detection of Sam 68 .
Western Blotting Analysis: 1:500-1:10,000 of a representative lot detected Sam 68 in RIPA lysates from HeLa cells.
Immunohistochemistry Analysis: A 1:600 dilution from a representative lot detected Sam 68 in mouse colon tissue (Data courtesy of Stephane Richard, McGill University).
Western Blotting Analysis: A representative lot detected Sam 68 in endogenous Sam 68 and exogenously expressed GFP-Sam 68 fusion in HeLa cells (Chen, T., et al. (1999). Mol Biol Cell. 10(9):3015-3033).
Western Blotting Analysis: A representative lot detected Sam 68 in HEK293 transfected with GFP-Sam68, mouse cortex, hippocampus, cerebellum, and mouse brain (Iijima, T., et al. (2011). Cell. 147(7):1601-1614).
Western Blotting Analysis: A representative lot detected Sam 68 in HeLa cells transfected with GFP-Sam68, MDA-MB-231, MDA-MB-468, and BT20 (Lukong, K.E., et al. (2005). J Biol Chem. 280(46):38639-38647).
Western Blotting Analysis: A representative lot detected Sam 68 in lysates from isolated mouse cells and mouse embryonic cell (Richard, S., et al. (2005). PLoS Genet. 1(6):e47).
Immunohistochemistry Analysis: A representative lot detected Sam 68 in mouse cerebellum, parasagittal section of brain stem and cerebellum (Iijima, T., et al. (2011). Cell. 147(7):1601-1614).
Immunohistochemistry Analysis: A representative lot detected Sam 68 in embryonic mice (Richard, S., et al. (2005). PLoS Genet. 1(6):e47).
Immunocytochemistry Analysis: A representative lot detected Sam 68 in HeLa cells (Chen, T., et al. (1999). Mol Biol Cell. 10(9):3015-3033).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Sam 68 in HeLa, HeLa cells transfected with GFP-Sam68, and MDA-MB-468 (Lukong, K.E., et al. (2005). J Biol Chem. 280(46):38639-38647).
Immunohistochemistry Analysis: A 1:600 dilution from a representative lot detected Sam 68 in mouse colon tissue (Data courtesy of Stephane Richard, McGill University).
Western Blotting Analysis: A representative lot detected Sam 68 in endogenous Sam 68 and exogenously expressed GFP-Sam 68 fusion in HeLa cells (Chen, T., et al. (1999). Mol Biol Cell. 10(9):3015-3033).
Western Blotting Analysis: A representative lot detected Sam 68 in HEK293 transfected with GFP-Sam68, mouse cortex, hippocampus, cerebellum, and mouse brain (Iijima, T., et al. (2011). Cell. 147(7):1601-1614).
Western Blotting Analysis: A representative lot detected Sam 68 in HeLa cells transfected with GFP-Sam68, MDA-MB-231, MDA-MB-468, and BT20 (Lukong, K.E., et al. (2005). J Biol Chem. 280(46):38639-38647).
Western Blotting Analysis: A representative lot detected Sam 68 in lysates from isolated mouse cells and mouse embryonic cell (Richard, S., et al. (2005). PLoS Genet. 1(6):e47).
Immunohistochemistry Analysis: A representative lot detected Sam 68 in mouse cerebellum, parasagittal section of brain stem and cerebellum (Iijima, T., et al. (2011). Cell. 147(7):1601-1614).
Immunohistochemistry Analysis: A representative lot detected Sam 68 in embryonic mice (Richard, S., et al. (2005). PLoS Genet. 1(6):e47).
Immunocytochemistry Analysis: A representative lot detected Sam 68 in HeLa cells (Chen, T., et al. (1999). Mol Biol Cell. 10(9):3015-3033).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Sam 68 in HeLa, HeLa cells transfected with GFP-Sam68, and MDA-MB-468 (Lukong, K.E., et al. (2005). J Biol Chem. 280(46):38639-38647).
品質
Evaluated by Western Blotting in MDA-MB-468 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Sam68 in 10 µg of MDA-MB-468 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Sam68 in 10 µg of MDA-MB-468 cell lysate.
ターゲットの説明
~68 kDa observed
関連事項
Replaces: 07-415
物理的形状
Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
07-415-I:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
SAM68 regulates neuronal activity-dependent alternative splicing of neurexin-1.
Iijima, T; Wu, K; Witte, H; Hanno-Iijima, Y; Glatter, T; Richard, S; Scheiffele, P
Cell null
mRNAs associated with the Sam68 RNA binding protein.
Tremblay, GA; Richard, S
RNA Biology null
Tyrosine phosphorylation of sam68 by breast tumor kinase regulates intranuclear localization and cell cycle progression.
Lukong, KE; Larocque, D; Tyner, AL; Richard, S
The Journal of Biological Chemistry null
Stéphane Richard et al.
PLoS genetics, 1(6), e74-e74 (2005-12-20)
The Src substrate associated in mitosis of 68 kDa (Sam68) is a KH-type RNA binding protein that has been shown to regulate several aspects of RNA metabolism; however, its physiologic role has remained elusive. Herein we report the generation of
Idir Malki et al.
Nucleic acids research, 50(22), 13045-13062 (2022-12-21)
Sam68, also known as KHDRBS1, is a member of the STAR family of proteins that directly link signal transduction with post-transcriptional gene regulation. Sam68 controls the alternative splicing of many oncogenic proteins and its role is modulated by post-translational modifications
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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