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Key Documents

安全性情報

LDP-505

Sigma-Aldrich

Lyophilized Cationic Liposomes

DOTAP:DOPC (10:90 molar ratio)

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About This Item

UNSPSCコード:
12352211
NACRES:
NA.23

形状

lyophilized powder

組成

DOTAP:DOPC (10:90 molar ratio)

white to off-white

平均粒径

100 nm

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詳細

Lyophilized liposomes product series are freeze-dried liposomes with various types of lipids and wide range of zeta potentials and different properties. These products should be used by scientists who understand liposome formulation and have the proper equipment to check the size, separate non-encapsulated drugs and do the proper assays.
Liposomes are extensively used to study the interaction of proteins, peptides and other molecules with the surface of a lipid membrane. One of the parameters that affects this interaction is the charge of the liposomal membrane. Liposomes are always made in aqueous environment and they are sized to the desired size in liquid state using various methods such as high-pressure extrusion through nano sized pore track etch membranes. Liposome without water is meaningless. In rare occasions, liposomes are freeze dried and proliposomes are formed in the presence of a lyoprotectant such as trehalose. Using a lyoprotectant is necessary in order to maintain the size of the liposomes after rehydration.
DOTAP is a cationic lipid and the liposomes made from DOTAP are positively charged. The amount of the positive charge depends on the percentage of the DOTAP in the formulation. For many different types of experiments and especially the studies that involve the interaction of a protein or peptide with the surface of liposomes, charged liposomes (cationic or anionic) are used. Charged liposomes are also used for many different types of blood complement studies. Cationic liposomes are mainly used for delivery of genetic material.

アプリケーション

  • Liposomes are formed upon hydration of the lyophilized formulation. If the lyophilized liposomes are hydrated with solution containing a water-soluble drug, then a large percentage of the drug will stay outside of the liposomes and in non-encapsulated form. It is advised to use a micro dialysis cassette or a spin column using the right-side beads (depending on the size of your drug) and separate the drug encapsulated liposomes from free drug and perform the drug assay in order to calculate the encapsulation efficiency.
  • Lyophilized liposomes are mainly recommended to be used with drugs that have a short life in aqueous solution mainly due to hydrolysis. After adding the solution of the drug to lyophilized liposomes, the liposomes should be used immediately.
  • Lyophilized liposomes products should be used by scientists who understand liposome formulation and have the proper equipment in order to check the size, separate the non-encapsulated drug and do the proper assays.
  • Trehalose is used as a lyoprotectant in all freeze-dried liposome formulation. The size distribution after hydration of the freeze-dried formulation will be around 100 nm.
  • Freeze-dried liposomes should be kept at -20°C.

保管分類コード

13 - Non Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

LDP-505-10MG:
LDP-505-5MG:


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