TLC Analysis of Aflatoxin in walnut samples on HPTLC Silica gel 60 F254

Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
1.05628 HPTLC Silica gel 60 F 254 10 cm x 10 cm (1.05628.0001)
eluent
[A] chloroform; [B] acetone; (9:1, A:B)
migration distance
5 cm
chamber
normal chamber without chamber saturation
detector
UV 366 nm and scan with TLC Scanner III (CAMAG)
Description
Application
Sample Prep: 100 μl walnut extract was spiked with 400 μl Aflatoxin standard. Application by spraying 6 mm bands with CAMAG ATS4