LC/MS/MS Analysis of Polymeric Isocyanates as Their MDI-DBA Derivatives on Ascentis® Express C18 after Collection using ASSET® EZ4-NCO Dry Sampler
Materials
analytical column
sample collection
standard
MDI-DBA Oligomers Standard
certified reference material, in methanol (varied conc.), ampule of 1 mLCONDITIONS
sample preparation
Solvent Desorption
sample/matrix
ambient air
adsorbent tube
ASSET EZ4-NCO Dry Sampler (5028-U)
extraction
Contact Sigma-Aldrich Technical Services for extraction and full analytical protocol.
column
Ascentis Express C18, 5 cm x 2.1 mm I.D., 2 μm particles (50811-U)
mobile phase
[A] 95:5 (0.05% formic acid in water):(0.05% formic acid in acetonitrile); [B] 95:5 (0.05% formic acid in acetonitrile):(0.05% formic acid in water)
gradient
60% to 80% B in 2 min, held for 3 min, to 100% B in 0.1 min, held for 9.9 min, to 60% B in 0.1 min, held for 3.9 min
flow rate
0.4 mL/min
pressure
3626 psi (250 bar)
column temp.
35 °C
detector
MS/MS, ESI(+), MRM
injection
2.0 μL
sample
ASSET sampler filter spike, MDI monomer (7.37 μg/mL), MDI 3-ring oligomer (0.284 μg/mL), MDI 4-ring oligomer (0.912 μg/mL)
Description
Analysis Note
This application demonstrates the simultaneous analysis of dibutylamine derivatized isocyanate MDI monomer and oligomers.
Other Notes
ASSET filters were spiked at various concentrations prior to extraction.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Asset is a registered trademark of Merck KGaA, Darmstadt, Germany