HPLC Analysis of Tryptic Peptides from Ovalbumin on SeQuant^® ZIC-HILIC

column

SeQuant ZIC-HILIC, PEEK, 150 x 0.3 mm, 5 μm, 200 Å (1.50481.0001)

mobile phase

[A] 0.25% (v/v) formic acid with Milli-q-water; [B] 0.25% (v/v) formic acid in acetonitrile; (10:90, A:B)

gradient

Initial composition: 90% B and 10% A. Linear gradient from 0-40 min

flow rate

5 μL/min

detector

MicroMass Ultima-QTOF, Needle voltage: 3.1 kV, Cone voltage: 45V (collision energy: 10V; Scan range: m/z 400-1800; 1.5 s cycle time)

injection

1 μL of 10 pmole digested sample injected in mobile phase