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  • Phosphatidylinositol transfer protein-α in platelets is inconsequential for thrombosis yet is utilized for tumor metastasis.

Phosphatidylinositol transfer protein-α in platelets is inconsequential for thrombosis yet is utilized for tumor metastasis.

Nature communications (2017-11-01)
Liang Zhao, Chelsea L Thorsheim, Aae Suzuki, Timothy J Stalker, Sang H Min, Lurong Lian, Gregory D Fairn, Shamshad Cockcroft, Amy Durham, Sriram Krishnaswamy, Charles S Abrams
ABSTRACT

Platelets are increasingly recognized for their contributions to tumor metastasis. Here, we show that the phosphoinositide signaling modulated by phosphatidylinositol transfer protein type α (PITPα), a protein which shuttles phosphatidylinositol between organelles, is essential for platelet-mediated tumor metastasis. PITPα-deficient platelets have reduced intracellular pools of phosphoinositides and an 80% reduction in IP

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
RIPA Buffer
Sigma-Aldrich
Pluronic® F-127, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Red Blood Cell Lysing Buffer Hybri-Max, liquid, sterile-filtered, suitable for hybridoma