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  • Characterization of Proliferating Neural Progenitors after Spinal Cord Injury in Adult Zebrafish.

Characterization of Proliferating Neural Progenitors after Spinal Cord Injury in Adult Zebrafish.

PloS one (2015-12-03)
Subhra Prakash Hui, Tapas Chandra Nag, Sukla Ghosh
ABSTRACT

Zebrafish can repair their injured brain and spinal cord after injury unlike adult mammalian central nervous system. Any injury to zebrafish spinal cord would lead to increased proliferation and neurogenesis. There are presences of proliferating progenitors from which both neuronal and glial loss can be reversed by appropriately generating new neurons and glia. We have demonstrated the presence of multiple progenitors, which are different types of proliferating populations like Sox2+ neural progenitor, A2B5+ astrocyte/ glial progenitor, NG2+ oligodendrocyte progenitor, radial glia and Schwann cell like progenitor. We analyzed the expression levels of two common markers of dedifferentiation like msx-b and vimentin during regeneration along with some of the pluripotency associated factors to explore the possible role of these two processes. Among the several key factors related to pluripotency, pou5f1 and sox2 are upregulated during regeneration and associated with activation of neural progenitor cells. Uncovering the molecular mechanism for endogenous regeneration of adult zebrafish spinal cord would give us more clues on important targets for future therapeutic approach in mammalian spinal cord repair and regeneration.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Vimentin Antibody, clone V9, clone V9, Chemicon®, from mouse
Sigma-Aldrich
Anti-A2B5 Antibody, clone A2B5-105, clone A2B5-105, Chemicon®, from mouse
Sigma-Aldrich
Anti-CNPase Antibody, clone 11-5B, clone 11-5B, Chemicon®, from mouse
Sigma-Aldrich
Anti-Oct-4 Antibody, Chemicon®, from rabbit
Sigma-Aldrich
Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, Chemicon®, from rabbit