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  • Enhancement of Palmarumycin C12 and C13 Production by the Endophytic Fungus Berkleasmium sp. Dzf12 in an Aqueous-Organic Solvent System.

Enhancement of Palmarumycin C12 and C13 Production by the Endophytic Fungus Berkleasmium sp. Dzf12 in an Aqueous-Organic Solvent System.

Molecules (Basel, Switzerland) (2015-11-17)
Yan Mou, Dan Xu, Ziling Mao, Xuejiao Dong, Fengke Lin, Ali Wang, Daowan Lai, Ligang Zhou, Bingyan Xie
ABSTRACT

The endophytic fungus Berkleasmium sp. Dzf12, isolated from Dioscorea zingiberensis, was found to produce palmarumycins C12 and C13 which possess a great variety of biological activities. Seven biocompatible water-immiscible organic solvents including n-dodecane, n-hexadecane, 1-hexadecene, liquid paraffin, dibutyl phthalate, butyl oleate and oleic acid were evaluated to improve palmarumycins C12 and C13 production in suspension culture of Berkleasmium sp. Dzf12. Among the chosen solvents both butyl oleate and liquid paraffin were the most effective to improve palmarumycins C12 and C13 production. The addition of dibutyl phthalate, butyl oleate and oleic acid to the cultures of Berkleasmium sp. Dzf12 significantly enhanced palmarumycin C12 production by adsorbing palmarumycin C12 into the organic phase. When butyl oleate was fed at 5% (v/v) in medium at the beginning of fermentation (day 0), the highest palmarumycin C12 yield (191.6 mg/L) was achieved, about a 34.87-fold increase in comparison with the control (5.3 mg/L). n-Dodecane, 1-hexadecene and liquid paraffin had a great influence on the production of palmarumycin C13. When liquid paraffin was added at 10% (v/v) in medium on day 3 of fermentation, the palmarumycin C13 yield reached a maximum value (134.1 mg/L), which was 4.35-fold that of the control (30.8 mg/L). Application of the aqueous-organic solvent system should be a simple and efficient process strategy for enhancing palmarumycin C12 and C13 production in liquid cultures of the endophytic fungus Berkleasmium sp. Dzf12.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dibutyl phthalate, 99%
Sigma-Aldrich
Hexadecane, anhydrous, ≥99%
Sigma-Aldrich
Hexadecane, ReagentPlus®, 99%
Sigma-Aldrich
Dibutyl phthalate, SAJ special grade, ≥98.0%
Sigma-Aldrich
Dodecane, anhydrous, ≥99%
Sigma-Aldrich
Oleic acid, technical grade, 90%
Sigma-Aldrich
1-Hexadecene, ≥98.5%
Sigma-Aldrich
Oleic acid, BioReagent, suitable for cell culture
Sigma-Aldrich
Sodium phosphate dibasic dodecahydrate, JIS special grade, ≥99.0%
Sigma-Aldrich
Sodium phosphate dibasic dodecahydrate, SAJ first grade, ≥98.0
Sigma-Aldrich
Oleic acid, SAJ first grade, ≥70.0%
Sigma-Aldrich
DBP, 98% (HPLC)
Sigma-Aldrich
Oleic acid, natural, FCC
Sigma-Aldrich
Oleic acid, ≥99% (GC)
Sigma-Aldrich
Sodium phosphate dibasic dodecahydrate, BioXtra, ≥99.0% (T)
Sigma-Aldrich
Sodium phosphate dibasic dodecahydrate, meets analytical specification of Ph. Eur., BP, E339, 98.5-102.5% (T)
Sigma-Aldrich
Oleic acid, meets analytical specification of Ph, Eur., 65.0-88.0% (GC)
Sigma-Aldrich
Sodium phosphate dibasic dodecahydrate, puriss. p.a., crystallized, ≥99.0% (T)
Sigma-Aldrich
Dodecane, ReagentPlus®, ≥99%