- A novel functional role for MMSET in RNA processing based on the link between the REIIBP isoform and its interaction with the SMN complex.
A novel functional role for MMSET in RNA processing based on the link between the REIIBP isoform and its interaction with the SMN complex.
PloS one (2014-06-14)
Fabio Mirabella, Alexander Murison, Lauren I Aronson, Christopher P Wardell, Andrew J Thompson, Sarah J Hanrahan, Jacqueline H L Fok, Charlotte Pawlyn, Martin F Kaiser, Brian A Walker, Faith E Davies, Gareth J Morgan
PMID24923560
ABSTRACT
The chromosomal translocation t(4;14) deregulates MMSET (WHSC1/NSD2) expression and is a poor prognostic factor in multiple myeloma (MM). MMSET encodes two major protein isoforms. We have characterized the role of the shorter isoform (REIIBP) in myeloma cells and identified a clear and novel interaction of REIIBP with members of the SMN (survival of motor neuron) complex that directly affects the assembly of the spliceosomal ribonucleic particles. Using RNA-seq we show that REIIBP influences the RNA splicing pattern of the cell. This new discovery provides novel insights into the understanding of MM pathology, and potential new leads for therapeutic targeting.
MATERIALS
Product Number
Brand
Product Description
Alanine, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Formaldehyde solution, JIS special grade, 36.0-38.0%, contains methanol as stabilizer
Sigma-Aldrich
Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Supelco
Formaldehyde solution, stabilized with methanol, ~37 wt. % in H2O, certified reference material
Sigma-Aldrich
Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)