- Letter: Mass spectrometric approach of high pH- and copper-induced glutathione oxidation.
Letter: Mass spectrometric approach of high pH- and copper-induced glutathione oxidation.
European journal of mass spectrometry (Chichester, England) (2013-07-12)
Gabi Drochioiu, Laura Ion, Catalina Ciobanu, Laura Habasescu, Ionel Mangalagiu
PMID23841227
ABSTRACT
The interaction between copper ions and gamma-L-glutamyl-L-cysteinyl-glycine [glutathione (GSH)] molecules may lead to the formation of the physiologically occurring Cu[I)-[GSH]2 and Cu(II)-GSSG complexes. Since glutathione depletion in neurons and aberrant copper metabolism have been implicated in several neurodegenerative disorders, we studied here the interaction of GSH with copper ions (Cu2+) by electrospray ionization ion trap mass spectrometry (ESI-IT-MS). Besides, we extended this approach to pH in excess of 10 by adding ethanolamine to the solution being investigated. As a result, the ESI-IT-MS spectra revealed novel aspects regarding the speciation of copper-glutathione complex.
MATERIALS
Product Number
Brand
Product Description
Continuous cast copper (O), BCR®, certified reference material, rod
Copper (S, P), BCR®, certified reference material, chips
Copper, IRMM®, certified reference material, 0.5 mm wire
Copper, IRMM®, certified reference material, 0.1 mm foil