- Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and ω-transaminase.
Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and ω-transaminase.
Organic & biomolecular chemistry (2012-02-22)
Young-Man Seo, Sam Mathew, Han-Seop Bea, Yong-Ho Khang, Sang-Hyeup Lee, Byung-Gee Kim, Hyungdon Yun
PMID22344532
ABSTRACT
A deracemization method was developed to generate optically pure L-homoalanine from racemic homoalanine using D-amino acid oxidase and ω-transaminase. A whole cell reaction using a biphasic system converted 500 mM racemic homoalanine to 485 mM L-homoalanine (>99% ee).