TOX8

In Vitro Toxicology Assay Kit, Resazurin based

• Synonym(s): Fluorescent resazurin toxicology assay
• EC Number: 263-718-5
• UNSPSC Code: 12352207
• NACRES: NA.84

Properties

• form: liquid
• usage: 1 kit sufficient for 2,000 tests
• packaging: pkg of 1 kit
• storage condition: dry at room temperature
• technique(s): protein quantification: suitable
• application(s): cell analysis; detection
• detection method: colorimetric; fluorometric
• storage temp.: 2-8°C

Description

General description

In Vitro Toxicology Assay Kit (Resazurin based) is designed for fluorometrically or spectrophotometrically determining cell number as a function of metabolic activity using the dye resazurin. The resazurin method is simple, accurate, and reproducible

Application

In Vitro Toxicology Assay Kit, Resazurin based has been used:

• in cell viability assay of human primary fibroblasts
• to determine the cell proliferation rate in murine pre-osteoblasts
• to determine the metabolic activity of microfluidic and monolayer cultures

Biochem/physiol Actions

Bioreduction of the dye reduces the amount of its oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable