SEP020

Seppro^® IgY14

LC2

• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• column D × L: 6.4 mm × 63.0 mm
• capacity: 2.5 mg total protein loading(35 μL of human plasma, average human protein concentration 70 mg/mL)
• storage temp.: 2-8°C

Description

General description

The targeted highly abundant proteins are simultaneously removed by the immobilized specific IgYs when crude biological samples are passed through the column. Selective immunodepletion provides an enriched pool of low abundance proteins for downstream proteomic analyses. Specific removal of these 14 highly abundant proteins depletes ~95% of the total protein mass from human serum or plasma. The low abundance proteins in the flow-through fractions can then be studied. Removal of highly abundant proteins enables improved resolution and dynamic range for one dimensional electrophoresis (1DGE), two-dimensional (2DGE) electrophoresis and liquid chromatography/ mass spectrometry (LC/MS). The collected flow-through fractions may need to be concentrated dependent upon the downstream application.

Seppro^® IgY14 columns allow users to separate 14 highly abundant proteins (HAP) from plasma protein mixtures. The Avian polyclonal IgY (Immunoglobulin Yolk) antibodies, have a distinct, highly specific partitioning advantage over competing products, allowing the researcher to look at a much cleaner human protein mixture. Seppro^® columns make further proteomics analysis into the plasma proteome much easier and provide cleaner results.

Proteins Depleted:

Albumin
IgG
α1-Antitrypsin
IgA
IgM
Transferrin
Haptoglobin
α2-Macroglobulin
Fibrinogen
Complement C3
α1-Acid Glycoprotein (Orosomucoid)
HDL (Apolipoproteins A-I and A-II)
LDL (mainly Apolipoprotein B)

Application

Seppro^® IgY14, LC2 (2 mL bed volume columns) is designed for use as a plasma/serum protein removal tool base upon immune affinity removal of 14 highly abundant proteins (HAP) from human plasma protein mixtures via liquid chromatography. Seppro^® IgY14 is used to support the analysis of low abundance human serum/plasma proteins.

Storage and Stability

Store the column at 2–8 °C. After use, equilibrate the column with 1´ Dilution Buffer containing 0.02% sodium azide and store the column at 2–8 °C with the end-caps tightly sealed. Do Not Freeze the column.

Other Notes

If the protein concentration of the sample is unknown and the total serum protein level is potentially elevated, a reduction of the serum load to 24.0 ml is recommended for initial study to avoid potential abundant protein bleed through.

Legal Information

Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

• S4199 Seppro^® Dilution buffer
• S4324 Seppro^® Stripping Buffer
• S4449 Seppro^® Neutralization Buffer
• CLS8163 Corning^® Costar^® Spin-X^® centrifuge tube filters, cellulose acetate membrane, pore size 0.45 μm, non-sterile

Safety Information

• Storage Class Code: 10 - Combustible liquids