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Safety Information

S150-05F

Sigma-Aldrich

Human Skeletal Muscle Cells: HSkMC: Pre-Screened for Insulin & AMPK signaling, fetal

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About This Item

UNSPSC Code:
12352204

biological source

human skeletal muscle (normal limb)

packaging

pkg of 500,000 cells

growth mode

Adherent

karyotype

2n = 46

morphology

Skeletal muscle

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

muscular dystrophy

shipped in

dry ice

storage temp.

−196°C

General description

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Our pre-screened Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of hamstrings and retain morphological, biochemical, and metabolic characteristics of skeletal muscle. Pre-screened HSkMCs can undergo differentiation to exhibit actin and myosin myofilaments and are specially tested for functional AMPK and Insulin Signaling Pathways.

1. AMPK Signaling Pathway
AMP-activated protein kinase (AMPK) is a major cellular regulator of lipid and glucose metabolism and mediates the metabolic changes associated with exercise. AMPK phosphorylates and inhibits activity of acetyl CoA carboxylase (ACC), the enzyme responsible for making malonyl-CoA which is required for fatty acid chain elongation.

2. Insulin Signaling Pathway
Skeletal muscle is one of major target tissue of insulin action. Upon insulin binding, insulin receptor tyrosine kinases catalyze autophosphorylation of tyrosine residues providing docking sites for downstream signaling components, such as IRS-1 and Grb2, which relay the signaling further into the cell (see Fig.B).

Cell Line Origin

Muscle

Application

lipid and glucose metabolism, exercise, insulin action, cell signaling

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 15 doublings

Subculture Routine

Please refer to the HSkMC Culture Protocol.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

S150-05F:

Certificates of Analysis (COA)

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