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RAB0448

Sigma-Aldrich

Cell-Based ELISA for detecting phospho-STAT4 (pTyr693) in cultured cell lines

96 assays

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.84

usage

96 assays

species reactivity

mouse, human

technique(s)

ELISA: suitable
capture ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... STAT4(6775)
mouse ... STAT4(20849)

General description

The Cell-Based Stat4 (pTyr693) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Stat4 (pTyr693) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human and mouse cell lines.

Immunogen

Stat4 (pTyr693) synthetic phosphopeptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABBLOCK5x Blocking Solution (Item F)SDS
  • RABFIX1Fixing Solution (Item D)SDS
  • RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)SDS
  • RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)SDS
  • RABPLATE1Uncoated MicroplateSDS
  • RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS
  • RABSTOP1Stop SolutionSDS
  • RABTMB1TMB Substrate Reagent (Item J)SDS
  • RABWASH120X Wash Buffer Concentrate ASDS
  • RABWASH220X Wash Buffer Concentrate BSDS
See All (10)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

Please refer to KIT Component information

FSL

Please refer to KIT Component information

ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

Please refer to KIT Component information

Cartagena Act

Please refer to KIT Component information

JAN Code

キットコンポーネントの情報を参照してください

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C M Bacon et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(16), 7307-7311 (1995-08-01)
Interleukin 12 (IL-12) is an important immunoregulatory cytokine whose receptor is a member of the hematopoietin receptor superfamily. We have recently demonstrated that stimulation of human T and natural killer cells with IL-12 induces tyrosine phosphorylation of the Janus family
K Yamamoto et al.
Molecular and cellular biology, 14(7), 4342-4349 (1994-07-01)
Interferon regulation of gene expression is dependent on the tyrosine phosphorylation and activation of the DNA-binding activity of two related proteins of 91 kDa (STAT1) and/or 113 kDa (STAT2). Recent studies have suggested that these proteins are substrates of Janus
Hua-Chen Chang et al.
The Journal of biological chemistry, 278(34), 32471-32477 (2003-06-14)
STAT4 (signal transducer and activator of transcription-4) mediates biological effects in response to interleukin-12 (IL-12). STAT4 has multiple domains that have distinct functions in signaling and gene activation. To characterize the role of the STAT4 N-terminal domain in mediating STAT4

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