R8029

Anti-RAD17 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-S.cerevisiae homolog of RAD24
• MDL number: MFCD08705510
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 70 kDa (also recognizes 75 kDa)
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): suitable; indirect immunofluorescence: 5-10 μg/mL using HEK293-T cells fixed with methanol-acetone; microarray: suitable; western blot: 0.5-1.0 μg/mL using HeLa cell lysates
• UniProt accession no.: O75943
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAD17(5884)

Description

General description

RFC-like DNA damage sensor (RAD17)/checkpoint clamp loader component, a homolog of the Schizosaccharomyces pombe checkpoint gene, rad17 is located on human chromosome 5q13.2. This gene is abundantly expressed in the testis.

Specificity

Anti RAD17 (C-terminal) specifically recognizes RAD17 (70 kDa and 75 kDa).

Immunogen

synthetic peptide corresponding to amino acids 623-640 of human Rad17 (isoform 2), conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is present in isoforms 1-4 of RAD17.

Application

Anti-RAD17 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

RFC-like DNA damage sensor (RAD17)/checkpoint clamp loader component participates in DNA replication, repair and modification or chromatin remodeling. Phosphorylation of RAD17 is a critical event during checkpoint signaling in DNA-damaged cells. RAD17 has been reported in colon, breast and non-small cell lung carcinoma. It may also participate in cell cycle control.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves