M5062

Anti-Myosin Va (LE-16) antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD03455612
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 190 kDa
• species reactivity: rat, chicken
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• concentration: ~0.5 mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections; microarray: suitable; western blot: 1:500 using a rat brain extract
• UniProt accession no.: Q02440
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MYO5A(4644) ; mouse ... Myo5a(17918) ; rat ... Myo5a(25017)

Description

General description

Myosin Va (p190) is a member of the unconventional class of myosins, distinct from both the myosins I and myosin II. It is present in neuronal and non neuronal cells of the brain. Class V myosins are widely expressed actin-based motors. Class V myosins have two motor head domains typical of myosins, and an extended regulatory neck domain with six tandem IQ domains that bind multiple calmodulin light chains. In addition, myosin V contains a unique 400 amino acids globular tail domain that may direct myosin V to its target or determine the cargo to which it binds.

Immunogen

synthetic peptide located near the C-terminus of chicken myosin Va (amino acids 1705-1720 with N-terminally added lysine) conjugated to KLH. This sequence is identical in human, mouse and rat.

Application

Anti-Myosin Va (LE-16) antibody produced in rabbit is used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Myosin Va is implicated in the regulation of vesicle trafficking in neurons and melanocytes. It regulates melanosome distribution along microfilaments. It is found in association with the centrosome at all stages of the cell cycle. In the interphase stage, myosin Va is found in pericentriolar material. During cell division, it is found in the cytoplasm and concentrates in a trail between migrating centrioles and in the mitotic spindle poles and spindle fibers.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable