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F5554

Sigma-Aldrich

Formalin, 10%, Neutral Buffered, Wintergreen Scented

histological fixative, pH 6.9-7.1

Synonym(s):

Formaldehyde, Methanal

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

shelf life

Expiry date on the label.

color

colorless

pH

6.90-7.10

application(s)

hematology
histology

storage temp.

room temp

General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative for light microscopy. 10% NBF is well suited for large throughput laboratories and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

Application

Formalin, 10%, Neutral Buffered, Wintergreen Scented has been used as a fixative in transformation and tumorigenicity assay and in immunohistostaining.
Intended for use as a general purpose histological fixative. Wintergreen oil has been added to the standard formulation to provide a wintergreen scent.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

PRTR

Specified Class I Designated Chemical Substances

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

F5554-BULK:
F5554-19L-PW:
F5554-4L:4548173295909
F5554-19L:4548173953090
F5554-200L:4548173277868
F5554-VAR:
F5554-4L-PW:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effects of formalin fixation on tissue optical
M F G Wood
Physics in Medicine and Biology, 56, V115-V115 (2011)
p38alpha MAP kinase as a sensor of reactive oxygen species in tumorigenesis
Dolado I, et al.
Cancer Cell, 11(2), 191-205 (2007)
Neither epithelial nor mesenchymal circulating tumor cells isolated from breast cancer patients are tumorigenic in NOD-scid Il2rg null mice
Donnenberg VS, et al.
NPJ breast cancer, 2, 16004-16004 (2016)
Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues
Magdeldin, et al.
Proteomics, 12(7), 1045-1058 (2012)
A method to estimate the formaldehyde concentration in fixative solutions
Simos, P
Pathology, 43, 394-394 (2001)
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