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E6280

Sigma-Aldrich

Anti-Endothelial Cell Protein C Receptor antibody, Rat monoclonal

clone RCR-252, purified from hybridoma cell culture

Synonym(s):

Anti-EPCR

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About This Item

MDL number:
MFCD04118502
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RCR-252, monoclonal

form

buffered aqueous solution

mol wt

antigen 49 kDa

species reactivity

human

technique(s)

flow cytometry: 5-20 μg/mL using HUVEC cells
microarray: suitable

isotype

IgG1

UniProt accession no.

Q9UNN8

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PROCR(10544)

Related Categories

General description

Anti-endothelial cell protein C receptor antibody, rat monoclonal (EPCR) (rat IgG1 isotype) is derived from the RCR-252 hybridoma produced by the fusion of mouse SP2/0 myeloma cells and cells isolated from the superficial inguinal lymph nodes from Wister rats immunized with human EPCR-positive RE-1 cells. Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor) is a protein encoded by the PROCR gene in humans. It is predominantly expressed in endothelial. The EPCR is also expressed in small vessels such as capillaries of the alveolar wall in the lung. EPCR is a member of the CD1/major histocompatibility complex superfamily.

Immunogen

human EPCR-positive RE-1 cells.

Application

Anti-endothelial cell protein C receptor antibody, rat monoclonal has been used in flow cytometry (FACS analysis) and in blocking the binding of the antigen presenting cell (APC) ligand to the EPCR.

Biochem/physiol Actions

Endothelial protein C receptor (EPCR) is involved in regulation of the cytoprotective and anticoagulant pathways of protein C. EPCR plays an important role in regulating the inflammatory response. It is also identified as an endothelial receptor for specific P. falciparum erythrocyte membrane protein 1 (PfEMP1) subtypes. EPCR is associated with an increased risk of venous thromboembolism (VTE).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

E6280-VAR:
E6280-BULK:
E6280-200UL:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mark R Gillrie et al.
Cellular microbiology, 17(12), 1883-1899 (2015-06-30)
Plasmodium falciparum-infected erythrocytes (IRBC) expressing the domain cassettes (DC) 8 and 13 of the cytoadherent ligand P. falciparum erythrocyte membrane protein 1 adhere to the endothelial protein C receptor (EPCR). By interfering with EPCR anti-coagulant and pro-endothelial barrier functions, IRBC adhesion
Marion Avril et al.
mBio, 7(4) (2016-07-14)
Intercellular adhesion molecule 1 (ICAM-1) and the endothelial protein C receptor (EPCR) are candidate receptors for the deadly complication cerebral malaria. However, it remains unclear if Plasmodium falciparum parasites with dual binding specificity are involved in cytoadhesion or different parasite
The endothelial cell protein C receptor (EPCR) functions as a primary receptor for protein C activation on endothelial cells in arteries, veins, and capillaries
Ye X, et al.
Biochemical and biophysical research communications, 259(3), 671-677 (1999)
Alice G Vassiliou et al.
Intensive care medicine, 39(10), 1752-1759 (2013-07-25)
Endothelial protein C receptor (EPCR) is expressed mainly in endothelial cells and is involved in regulation of the cytoprotective and anticoagulant pathways of protein C. We assessed whether haplotypes in the EPCR gene modify the risk of severe sepsis and/or
Maria Bernabeu et al.
mBio, 10(3) (2019-05-30)
Cerebral malaria is a severe neurological complication associated with sequestration of Plasmodium falciparum-infected erythrocytes (IE) in the brain microvasculature, but the specific binding interactions remain under debate. Here, we have generated an engineered three-dimensional (3D) human brain endothelial microvessel model
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