VH2
90102539, Daboia russelii cardiovascular , Fibroblast-like
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product name
VH2, from Daboia russelii cardiovascular, 90102539, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
biological source
Daboia russelii cardiovascular
growth mode
Adherent
karyotype
2n = 34
morphology
Fibroblast-like
receptors
Not specified
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Cell Line Origin
Viper Russells heart
Cell Line Description
Derived from the heart of a normal female Russell′s viper (Daboia russelii). Unlike cell line VSW the cells do not produce C type particles.
Culture Medium
EMEM (HBSS) (M5775) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + fetal bovine serum (FBS / FCS) (Heat Inactivated).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2, i.e., seeding at 3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 28-30°C.
Other Notes
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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