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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS1
Reactivity
Anti-CD45, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse NS1 x Mouse BALB/c spleen
Cell Line Description
The antibody reacts mainly with high molecular weight (190-220kDa) form of leukocyte common antigen. Labels all B lymphocytes and 50% of bone marrow cells and 2% of thymocytes. The antibody is directed against CD8+ve T, NK and 75% of CD4+ve cells. Same distribution as OX-32: antibodies do not compete.
Immunogen
PHA activated rat lymph node cells
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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