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S2890

Sigma-Aldrich

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

lyophilized powder, ≥700 DEA units/mg protein

Synonym(s):

Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii

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250 μG
¥25,200
1 MG
¥57,300

¥25,200

Estimated to ship onApril 11, 2025

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250 μG
¥25,200
1 MG
¥57,300

About This Item

MDL number:
MFCD00132384
UNSPSC Code:
12352203
NACRES:
NA.46

¥25,200

Estimated to ship onApril 11, 2025

Request a Bulk Order

conjugate

alkaline phosphatase conjugate

Quality Level

200

form

lyophilized powder

specific activity

≥700 DEA units/mg protein

composition

Protein, ≥40% biuret

storage temp.

−20°C

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General description

Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin.[1] Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.[2]

Application

Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.[3][4][5]
The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.

Unit Definition

One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

Physical form

Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

S2890-1MG-PW:
S2890-250UG:
S2890-BULK-N:
S2890-1MG:
S2890-PH:
S2890-VAR:
S2890PROC:
S2890-250UG-PW:
S2890-VAR-N:
S2890-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCN4419
SLCN4419
SLCL2931
SLCL2931
SLCF8013

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Effects of bovine Herpesvirus Type 5 on development of in vitro-produced bovine embryos.
Silva-Frade C
Theriogenology, 73(3), 324-331 (2010)
Bystander activation of irrelevant CD4+ T cells following antigen-specific vaccination occurs in the presence and absence of adjuvant
van Aalst S, et al.
PLoS ONE, 12(5), e0177365-e0177365 (2017)
An allosteric-probe for detection of alkaline phosphatase activity and its application in immunoassay
Guo J, et al.
Frontiers in Chemistry, 6, 618-618 (2018)
Production and characterization of virus-like particles and the P domain protein of GII.4 norovirus.
Koho T et al
Journal of Virological Methods, 179(1), 1-7 (2012)
Ola Blixt et al.
The Journal of biological chemistry, 278(33), 31007-31019 (2003-05-30)
Ten of the 11 known human siglecs or their murine orthologs have been evaluated for their specificity for over 25 synthetic sialosides representing most of the major sequences terminating carbohydrate groups of glycoproteins and glycolipids. Analysis has been performed using
View All Related Papers

Protocols

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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