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S2015

Sigma-Aldrich

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

Synonym(s):

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

sterility

0.2 μm filtered

form

solution

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

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General description

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Application

Suitable for:
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for microarrays

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Components

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

S2015-PH:
S2015PROC:
S2015-VAR:
S2015-1L-PW:
S2015-EW:
S2015-BULK:
S2015-1L:

Certificates of Analysis (COA)

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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