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62971

Sigma-Aldrich

Lysozyme from chicken egg white

powder (crystalline), 70000-140000 U/mg

Synonym(s):

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

CAS Number:
12650-88-3
Enzyme Commission number:
3.2.1.17 (BRENDA, IUBMB)
EC Number:
235-747-3
MDL number:
MFCD00131557
UNSPSC Code:
12352204
NACRES:
NA.22

biological source

chicken egg white

Quality Level

100

form

powder (crystalline)

specific activity

70000-140000 U/mg

mol wt

single-chain 14.3 kDa
Mr ~14600

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

UniProt accession no.

P00698

application(s)

cell analysis

storage temp.

2-8°C

Gene Information

chicken ... LYZ(396218)

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Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Unit Definition

1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

62971-50G-F:
62971-BULK-F:
62971-VAR-F:
62971-10G-F:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL9074
BCCL1874
BCCH3501
BCCG1994
BCCD6905

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