COUEDTAF-RO
Roche
cOmplete™ ULTRA Tablets, EDTA-free, glass vials Protease Inhibitor Cocktail
Tablets provided in glass vials
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.21
Recommended Products
form
tablet
usage
sufficient for 50 mL (05892953001)
sufficient for 50 mL (06538282001)
packaging
pkg of 2 × 10 tablets (05892953001)
pkg of 6 × 10 tablets (06538282001)
manufacturer/tradename
Roche
solubility
aqueous buffer: soluble
absorption
0.4 at 280 nm (1x solution)
storage temp.
2-8°C
General description
cOmplete ULTRA Tablets, EDTA-free contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. One cOmplete ULTRA Tablet, EDTA-free is sufficient for a volume of 50 ml extraction solution. cOmplete ULTRA Tablets contain both irreversible and reversible protease inhibitors. Tablets are supplied in glass vials. Alternatively, these tablets are also available in EASYpack blister packs. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer.
cOmplete ULTRA Tablets EDTA-free are identical to cOmplete ULTRA Tablets, the only difference being that no EDTA or other chelating agents (e.g. EGTA) are included.
Contents
Tablets in a glass vial. Each tablet is sufficient for a volume of 50ml extraction solution.
cOmplete ULTRA Tablets EDTA-free are identical to cOmplete ULTRA Tablets, the only difference being that no EDTA or other chelating agents (e.g. EGTA) are included.
Contents
Tablets in a glass vial. Each tablet is sufficient for a volume of 50ml extraction solution.
Specificity
Inhibits serine proteases, cysteine proteases. Does not inhibit metalloproteases.
Application
During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows high inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine and cysteine proteases. cOmplete ULTRA Tablets were developed for improved efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
This tablet does not contain EDTA; therefore metalloproteases are not inhibited.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine and cysteine proteases. cOmplete ULTRA Tablets were developed for improved efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
This tablet does not contain EDTA; therefore metalloproteases are not inhibited.
cOmplete™ ULTRA Tablets, EDTA-free, glass vials Protease Inhibitor Cocktail has been used as a component of
- ice-cold radioimmunoprecipitation assay buffer (RIPA) for the lysis of retinal ganglion cells
- lysis buffer for green fluorescent protein (GFP)- cell-penetrating peptides (CPPs) fusion protein isolation from E coli
- homogenization buffer for mice protein isolation from intestinal tissue
Features and Benefits
- Protect your precious proteins.
- Why settle for less.
- Defend against a broad range of proteases.
- Convenient and safe tablet format.
into your buffers.
Components
Proprietary mixture of several protease inhibitors with broad inhibitory specificity.
Physical form
Tablets in a glass vial. Each tablet is sufficient for a volume of 50 ml extraction solution.
Preparation Note
Working concentration: 1 Tablet per 50 ml extraction solution.
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One tablet is sufficient for the inhibition of proteolytic activity in 50 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 50 ml extraction buffer. The tablets can be added directly to the extraction medium.
Stock Solution (2x conc.)
Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add one cOmplete ULTRA Tablet, EDTA-free to 25 ml double-distilled water in a PE or PP tube.
• Vortex 4 min, until the tablet is dissolved.
• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• This stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.
Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One tablet is sufficient for the inhibition of proteolytic activity in 50 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 50 ml extraction buffer. The tablets can be added directly to the extraction medium.
Stock Solution (2x conc.)
Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add one cOmplete ULTRA Tablet, EDTA-free to 25 ml double-distilled water in a PE or PP tube.
• Vortex 4 min, until the tablet is dissolved.
• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• This stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.
Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
Reconstitution
Soluble in aqueous buffers, or add directly to extraction media. For the stock solution, use water only.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
cOmplete is a trademark of Roche
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible, corrosive hazardous materials
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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