MABT528

Anti-Connexin 43 Antibody, CT Antibody, clone 1C5.1

clone 1C5.1, from mouse

• Synonym(s): Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1C5.1, monoclonal
• species reactivity: rat, human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000156
• UniProt accession no.: P17302
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... GJA1(2697)

Description

General description

Gap junction alpha-1 protein (UniProt P17302; also known as Connexin-43, Cx43, Gap junction 43 kDa heart protein) is encoded by the GJA1 (also known as AVSD3, CMDR, GJAL, HSS, ODDD) gene (Gene ID 2697) in human. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Connexin-43 is a polytopic integral membrane protein oligomerizes into a hexamer or connexon. It is he most ubiquitously expressed connexin and contains a long C-terminal tail, an N-terminal domain, and multiple transmembrane domains. The C-terminal tail domain of human connexin-43 contains 50 amino acids and includes post-translational modification sites, as well as binding sites for transcription factors, cytoskeleton elements, and other proteins. The C-terminal tail regulates pH gating and channel assembly, whereas the N-terminal domain is involved in channel gating and oligomerization and controls the switch between channel open and closed states. Mutations in connexin-43 result in multiple disorders, such as oculodentodigital dysplasia that is characterized by microcornea, microphthalmia, and syndactyly of the fourth and fifth fingers. Connexin-43 is shown to negatively regulate growth of colon cancer cells, in part by enhancing apoptosis. Loss of connexin-43 expression in colorectal cancers is correlated with significantly shorter relapse-free and overall survival.

Ref.:
Sirnes, S et al. (2012). Int. J. Cancer 131, 570-581.
Naus, CC et al. (2010). Nat. Rev. Cancer. 10, 435-441.

Specificity

Clone 1C5.1 targets an epitope within the second half of the C-terminal cytoplasmic tail.

Immunogen

GST-tagged recombinant human connexin 43 C-terminal cytoplasmic tail fragment.

Application

Anti-Connexin 43, CT, clone 1C5.1, Cat. No. MABT528 is a mouse monoclonal antibody validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of connexin 43.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected cconnexin 43 in human cerebellum, heart muscle, and kidney tissue sections.

Quality

Evaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: 10 ng/mL of this antibody detected connexin 43 in 10 µg of HUVEC lysate.

Target description

~43 kDa observed. 42.88/42.90 kDa (human/rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: MAB3068

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1