MABS2173

Anti-PNPLA3/Adiponutrin Antibody, clone 19A6

• Synonym(s): 1-acylglycerol-3-phosphate O-acyltransferase Pnpla3;EC:2.3.1.51;Acylglycerol transacylase;Adiponutrin;ADPN;Calcium-independent phospholipase A2-epsilon;iPLA2-epsilon;Lysophosphatidic acid acyltransferase;Patatin-like phospholipase domain-containing protei
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 19A6, monoclonal
• mol wt: calculated mol wt 45.77 kDa; observed mol wt ~43 kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100 μg
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: NP_473429
• UniProt accession no.: Q91WW7
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... PFN2(5217)

Description

General description

1-acylglycerol-3-phosphate O-acyltransferase Pnpla3 ( UniProt: Q91WW7; also known s EC:2.3.1.51, Acylglycerol transacylase, Adiponutrin, ADPN, Calcium-independent phospholipase A2-epsilon, iPLA2-epsilon, Lysophosphatidic acid acyltransferase, Patatin-like phospholipase domain-containing protein 3) is encoded by the Pnpla3 (also known as Adpn) gene (Gene ID: 116939) in murine species. PNPLA3 is a single-pass membrane glycoprotein with a cytoplasmic domain (aa 1-42), a transmembrane domain (aa 43-63), and a lumenal domain (aa 64-413). Its expression is restricted to adipose tissue and is also observed in liver in response to high-sucrose diet. It is found in hepatic lipid droplets. PNPLA3 catalyzes coenzyme A-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), which is an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. For its activity, acyl donors are long chain (at least C16) fatty acyl-CoAs. PNPLA3 also exhibits low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities. Mutations in Pnpla3 gene have been linked to increased risk of hepatic steatosis. Catalytically defective forms of PNPLA3 with I148M or S47A mutations are reported to cause increased hepatic triglyceride accumulation of triglycerides (TG) in sucrose fed mice. PNPLA3 knockdown is reported to reduce TG content in mice overexpressing I148 form. (Ref.: BasuRay, S., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(19); 9521-9526; BasuRay, S., et al. (2017). Hepatology. 66(4); 1111-1124; Kumari, M., et al. (2012). Cell Metab. 15(5); 691-702).

Specificity

Clone 19A6 is a rabbit monoclonal antibody that detects 1-acylglycerol-3-phosphate O-acyltransferase Pnpla3 (PNPLA3). It targets an epitope within the internal region.

Immunogen

A peptide corresponding to 178 amino acids from the internal region of mouse PNPLA3/Adiponutrin.

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse brain tissue extracts.

Western Blotting Analysis: A 1:125 dilution of this antibody detected PNPLA3 in Mouse brain tissue extracts.

Tested Applications

Western Blotting Analysis: A representative lot detected PNPLA3 in Western Blotting applications (BasuRay, S., et al. (2017). Hepatology. 66(4): 1111-1124).

Immunoprecipitation Analysis: A representative lot immunoprecipitated PNPLA3 in Immunoprecipitation applications (BasuRay, S., et al. (2017). Hepatology. 66(4): 1111-1124).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-PNPLA3/Adiponutrin, clone 19A6, Cat. No. MABS2173, is a rabbit monoclonal antibody that detects PNPLA3/Adiponutrin and is tested for use in Immunoprecipitation and Western Blotting.

Physical form

Purified rabbit monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1