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Safety Information

MABE160

Sigma-Aldrich

Anti-E2F4 Antibody, clone LLF4-2

clone LLF4-2, from mouse

Synonym(s):

Transcription factor E2F4, E2F-4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LLF4-2, monoclonal

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001941

UniProt accession no.

Q16254

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... E2F4(1874)

General description

E2F4 is the most common E2F family member in a number of cell types. E2F4 deficiency has been shown to cause a decrease in the thickness of the gut epithelium, decrease in maturation of hematopoietic cell types and an increased rate of apoptosis, suggesting that E2F4 plays a role in normal maturation of several cell types. E2F4 has also been shown to be essential for the normal development of airway epithelium and early eye development. Other data has implicated E2F4 during normal heart development, specifically cardiomyocyte proliferation.

Immunogen

Histidine-tagged recombinant protein corresponding to human E2F4.

Application

Anti-E2F4 Antibody, clone LLF4-2 is a Mouse Monoclonal Antibody for detection of E2F4 also known as Transcription factor E2F4, E2F-4 & has been validated in WB & IHC.
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Kinross, K., et al. (2006). Blood. 108(3):886-895.)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected E2F4 on 10 µg of HeLa cell lysate.

Target description

Bands may be detected at ~45 and ~60 kDA

Physical form

Format: Purified
Protein G
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MABE160:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jun Wu et al.
International journal of biological sciences, 18(3), 956-969 (2022-02-18)
To explore the mechanism of the bone anabolic action of p27 deficiency, we first confirmed that osteoblast formation and osteogenesis were significantly increased in p27 deficient mice compared with their wild-type littermates. Microarray analysis of differential gene expression profiles, followed
Noelia López-Sánchez et al.
Molecular neurobiology, 59(5), 3016-3039 (2022-03-08)
Alzheimer's disease (AD) has a complex etiology, which requires a multifactorial approach for an efficient treatment. We have focused on E2 factor 4 (E2F4), a transcription factor that regulates cell quiescence and tissue homeostasis, controls gene networks affected in AD
Maria C Cuitiño et al.
Cell reports, 27(12), 3547-3560 (2019-05-28)
Orchestrating cell-cycle-dependent mRNA oscillations is critical to cell proliferation in multicellular organisms. Even though our understanding of cell-cycle-regulated transcription has improved significantly over the last three decades, the mechanisms remain untested in vivo. Unbiased transcriptomic profiling of G0, G1-S, and S-G2-M

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