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Safety Information

ES009-M

Sigma-Aldrich

Fetal Bovine Serum

US Origin, EmbryoMax ES Cell Qualified FBS, sterile-filtered, suitable for stem cell culture

Synonym(s):

FBS, FCS, Sera, Serum

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About This Item

UNSPSC Code:
12352207
eCl@ss:
26020100
NACRES:
NA.75

biological source

bovine fetus

Quality Level

400

sterility

sterile-filtered

product line

FBS Select

manufacturer/tradename

Specialty Media
EmbryoMax®

origin

USA origin

technique(s)

cell culture | stem cell: suitable

impurities

endotoxin, tested
 mycoplasma, tested
 virus, tested

storage temp.

−20°C

General description

The EmbryoMax ES Cell Qualified FBS is exclusively of US origin. Each lot is sterile filtered and mycoplasma, endotoxin, and virus tested. Additionally, each lot is prescreened for plating efficiency and maintenance of undifferentiated morphology of mouse embryonic stem cells. A Certificate of Analysis is available upon request for each lot of EmbryoMax ES Cell Qualified FBS.
Note: Precipitates may develop upon freezing and/or thawing of this product. If precipitates are present, filter product through a 0.2 micron filter. This occurrence does not impact product performance.
The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum (FBS).

Quality

Each lot is tested and qualified for use with mouse embryonic stem cells.

Linkage

Replaces: ES-009-D

Physical form

Liquid

Storage and Stability

Store at -20°C.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ES-009-B:
ES-009-C:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Protocols

ADCC Protocol using BioIVT Cryopreserved NK Effector Cells

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

Primary Human Spheroid-Qualified Plateable Hepatocyte Culture Protocol

Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

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Evaluating of Stericup® Quick Release Filtration Devices for Fetal Bovine Serum (FBS) in Cell Culture

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Primary Human Suspension Hepatocytes Culture Protocol

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Fetal Bovine Serum that Makes the Grade

Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.

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