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CBL188

Sigma-Aldrich

Anti-Clathrin Antibody, clone CHC5.9

clone CHC5.9, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

CHC5.9, monoclonal

species reactivity

bovine, amphibian, rat, pig, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgM

NCBI accession no.

NM_004859.3

UniProt accession no.

Q00610

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CLTC(1213)

Specificity

This antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian

Immunogen

Coated vesicles (clathrin) of bovine brain.

Application

Anti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH.
Marker for detection of receptor mediated endocytosis

Suitable for frozen sections

Western blotting KNOW SPECIES

Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Organelle & Cell Markers

Physical form

Format: Purified
The antibody was purified by gel filtration and is presented as 100μg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4.

Storage and Stability

Maintain at 2-8°C for up to 12 months from date of receipt.

Analysis Note

Control
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

CBL188:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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