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AB1632

Sigma-Aldrich

Anti-Nitric Oxide Synthase I Antibody

Chemicon®, from rabbit

Synonym(s):

NOS I, bNOS, nNOS

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

bovine, mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_000620.1

UniProt accession no.

P29475

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Nos1(18125)

Specificity

Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.

Immunogen

Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.

Application

Anti-Nitric Oxide Synthase I Antibody is an antibody against Nitric Oxide Synthase I for use in ELISA, WB, IH.
Immunohistochemistry: Use at 5 - 10 μg/mL on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:100 - 1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons.

Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.

ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress

Physical form

Peptide affinity-purified immunoglobulin. Supplied at a 1mg/mL solution in PBS, pH 7.4, containing 0.1% BSA.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated thawing and refreezing.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB1632:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Agustín España et al.
Experimental dermatology, 22(2), 125-130 (2013-02-01)
Pemphigus vulgaris (PV) is an autoimmune blistering skin disease characterized by suprabasal acantholysis produced as a consequence of desmoglein (Dsg) and non-Dsg autoantibodies binding to several targeting molecules localized on the membrane of keratinocytes. Nitric oxide (NO) may exert a
A Özen et al.
Anatomia, histologia, embryologia, 42(5), 384-393 (2013-02-16)
This study was aimed to determine staining intensity, cellular localization and distribution of the nitric oxide synthase (NOS) enzymes during the sexual cycle in the cow oviduct. Oviduct samples belonging to 20 cows, 10 of which were in the estrual
G Scala et al.
Anatomia, histologia, embryologia, 41(6), 428-435 (2012-04-18)
The reticular groove mucosa of adult cattle, buffalo and sheep was investigated by histochemical and immunocytochemical techniques. Intense NADPH-d staining was observed in the folds of the epithelium mucosa and at the bottom of the reticular groove in all domestic
Gaetano Scala et al.
Anatomical record (Hoboken, N.J. : 2007), 290(11), 1399-1412 (2007-10-12)
Choroid plexuses (CPs) play pivotal roles in many processes that establish, survey, and maintain the biochemical and cellular status of the central nervous system (CNS). Changes in the anatomy and physiology of CPs have been linked to several CNS diseases.
Mark R Griffiths et al.
Immunology, 155(3), 346-355 (2018-06-21)
Microglia and non-professional immune cells (endothelial cells, neurons) participate in the recognition and removal of pathogens and tissue debris in the injured central nervous system through major pro-inflammatory processes. However, the mechanisms involved in regulating these responses remain ill-characterized. We
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