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662098

Sigma-Aldrich

UbcH5c Conjugating Enzyme E2, Human, Recombinant, E. coli

Synonym(s):

UBD2D3, Ubiquitin-conjugating enzyme E2 D3

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About This Item

UNSPSC Code:
12352203

Assay

≥95% (SDS-PAGE)

Quality Level

100

form

solution

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

Full-length, recombinant, human UbcH5c expressed in E. coli. UbcH5c, an E2 ubiquitin ligase, is highly homologous to the yeast UbC4/5 family of E2 enzymes. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of transcription factors such as p53, NF-κB3, and c-Fos. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Brzovic, P.S., et al. 2006. Cell Cycle5, 2867.
Gehrke, S.G., et al. 2003 Blood.101, 3288.
Nuber, U., And Scheffner, M., 1999. J. Biol. Chem.274, 7576.
Schwarz, S.E., et al. 1998. J. Biol. Chem.273, 12148.
Jensen, J., et al. 1995. J. Biol. Chem.270, 30408.
Scheffner, M., et al. 1994. Proc. Natl. Acad. Sci.91, 8797.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

662098-50UG:
662098-VUG:
662098-UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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