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T3073

Sigma-Aldrich

Tissue Preparation Solution

Tissue Preparation Solution

30 mL sufficient for 1,000 extractions

Synonym(s):

Direct PCR tissue preparation solution, Extract-N-Amp™ Tissue Preparation Solution, REDExtract-N-Amp™ Tissue Preparation Solution, SYBR® Green Extract-N-Amp™ Tissue Preparation Solution

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30 ML
¥76,400

¥76,400

In StockDetails

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30 ML
¥76,400

About This Item

EC Number:
254-457-8
UNSPSC Code:
12352200
NACRES:
NA.55

¥76,400

In StockDetails

Quality Level

200

usage

30 mL sufficient for 1,000 extractions
 sufficient for 1000 reactions

shipped in

wet ice

storage temp.

−20°C

General description

The Tissue Preparation Solution is intended for use with the Extract-N-Amp™ Tissue PCR kits, REDExtract-N-Amp™ Tissue PCR kits, and SYBR® Green Extract-N-Amp Tissue PCR kits. This product is intended to supplement the kit reagents, where there are many extractions.

Application

Tissue preparation solution has been used:
  • in combining segments of tails for genotyping[1]
  • to extract genomic DNA from zebrafish embryos/fin tissues[2]
  • to extract DNA from ear tags, tail tips and embryonic yolk sacs of mouse embryo[3]
  • for extraction of DNA from fungal spores and mycelia[4]

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

related product

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XNAT

REDExtract-N-Amp™ Tissue PCR Kit, sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

R4775

REDExtract-N-Amp™ PCR ReadyMix™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E8155

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E7526

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S4320

SYBR® Green Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

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SYBR® Green Extract-N-Amp™ Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNATRG

SYBR® Green Extract-N-Amp™ Tissue PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

T3073PROC:
T3073-VAR:
T3073-.3ML:
T3073-30ML-CO:
T3073-30ML:
T3073-BULK:
T3073-3ML-KC:
T3073-30ML-KC:
T3073-.3ML-KC:
T3073-3ML:

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Certificates of Analysis (COA)

Lot/Batch Number
0000402405
0000402404
0000393260
0000393257
0000369922

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Titilayo D O Falade et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 33(7), 1241-1253 (2016-06-07)
In vitro experimental environments are used to study interactions between microorganisms, and to predict dynamics in natural ecosystems. This study highlights that experimental in vitro environments should be selected to match closely the natural environment of interest during in vitro
N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.
Soh BS
Cell Research, 24(12), 1420-1432 (2014)
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Jessica Wallingford et al.
Frontiers in molecular neuroscience, 10, 268-268 (2017-09-14)
Astrocyte dysfunction has been indicated in many neurodevelopmental disorders, including Fragile X Syndrome (FXS). FXS is caused by a deficiency in fragile X mental retardation protein (FMRP). FMRP regulates the translation of numerous mRNAs and its loss disturbs the composition
K D Kuhn et al.
Molecular and cellular neurosciences, 103, 103467-103467 (2020-02-01)
During neural development, complex organisms rely on progressive and regressive events whereby axons, synapses, and neurons are overproduced followed by selective elimination of a portion of these components. Tumor necrosis factor α (TNFα) together with its cognate receptor (Tumor necrosis

Protocols

REDExtract-N-Amp™ Tissue PCR Kit Protocol

Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

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