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P8170

Sigma-Aldrich

Protein G−Peroxidase from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

Synonym(s):

Protein G−HRP from Streptococcus sp.

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About This Item

MDL number:
MFCD00165575
UNSPSC Code:
12352203
NACRES:
NA.46

recombinant

expressed in unspecified host

Quality Level

200

conjugate

peroxidase conjugate

form

lyophilized powder

technique(s)

direct ELISA: 1:100,000 using human IgG

storage temp.

−20°C

General description

Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains. Peroxidase from Streptococcus sp. is used to maintain the H2O2 homeostasis in cells. It aids the regulation of ABC Mn2+-permease complex (psaBCA) genes.

Specificity

The product binds IgG for most mammalian species (excluding feline) and can be used in ELISA or immunohistochemical formats. Protein was determined to be at 250-350ug per 250ug vial by UV absorbance. Reactive to chicken IgG as well.

Application

Protein G- Peroxidase from Streptococcus sp. has been used in indirect ELISA and immunoblotting.

Preparation Note

Labeled with peroxidase type VI by a modification of the procedure of O′Sullivan, M.S., et al., FEBS Lett., 95, 311 (1978) which favors low molecular weight conjugates. Conjugate is purified by gel filtration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Evaluation of a modified Rose Bengal test and an indirect enzyme-linked immunosorbent assay for the diagnosis of Brucella melitensis infection in sheep
Ferreira AC, et al.
Veterninary Research, 34(3), 297-305 (2003)
Thiol peroxidase is an important component of Streptococcus pneumoniae in oxygenated environments
Hajaj B, et al.
Infection and Immunity, IAI-00126 (2012)
Dimitra Zarafeta et al.
Frontiers in microbiology, 7, 1779-1779 (2016-12-03)
Lipolytic enzymes that retain high levels of catalytic activity when exposed to a variety of denaturing conditions are of high importance for a number of biotechnological applications. In this study, we aimed to identify new lipolytic enzymes, which are highly
Purification and some properties of streptococcal protein G, a novel IgG-binding reagent.
Bjorck L and Kronvall G
Journal of Immunology, 133(2), 969-974 (1984)
Moran Elbaz et al.
The Journal of biological chemistry, 295(30), 10331-10339 (2020-06-06)
Mutations in the ryanodine receptor 1 (RYR1) gene are associated with several human congenital myopathies, including the dominantly inherited central core disease and exercise-induced rhabdomyolysis, and the more severe recessive phenotypes, including multiminicore disease, centronuclear myopathy, and congenital fiber type
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Protocols

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

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