Skip to Content
Merck
All Photos(1)

Key Documents

DFF100

Sigma-Aldrich

DEAE–Sepharose

Fast Flow

Synonym(s):

Diethylaminoethyl–Sepharose

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
MDL number:
UNSPSC Code:
47101511
NACRES:
NA.56

Quality Level

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

pH

2—12

capacity

110-160 μeq/mL binding capacity(gel volume)

Looking for similar products? Visit Product Comparison Guide

General description

DFF100-500Ml′s update product number is GE17-0709-01

Application

DEAE-Sepharose has been used in:
  • anion exchange chromatography
  • to screen polyisoprenyl phosphate phosphatase activity
  • to purify isoinhibitors
  • for the purification of human immunodeficiency virus (HIV)
  • glycoprotein envelope (gp140 env)

DEAE-Sepharose is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.

Legal Information

Sepharose is a trademark of Cytiva

replaced by

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F

Flash Point(C)

38 - 43 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ahmet Mavi et al.
Journal of enzyme inhibition and medicinal chemistry, 21(2), 235-239 (2006-06-23)
The inhibition and activation effects of some drugs on the activities of superoxide dismutase enzymes (SOD) in human erythrocyte and leukocyte cells was investigated. Firstly, CuZnSOD enzyme was purified 837-fold and 12% efficiency from human erythrocytes by ethanol-chloroform treatment to
Identification and stability of trypsin inhibitor isoforms in pea (<I>Pisum sativum</I> L.) cultivars grown in New Zealand.
Morrison, S.C., et al.
Food Chemistry, 100(1), 1-7 (2007)
T M Schmidt et al.
Applied and environmental microbiology, 55(10), 2607-2612 (1989-10-01)
Luminescence of batch cultures of Xenorhabdus luminescens was maximal when cultures approached stationary phase; the onset of in vivo luminescence coincided with a burst of synthesis of bacterial luciferase, the enzyme responsible for luminescence. Expression of luciferase was aldehyde limited
Advances in Potato Chemistry and Technology (2016)
Growth cone collapse and neurite retraction from cultured <I>Helisoma</I> neurons in response to antibody Fab fragments against an extracellular matrix protein.
Miller, J.D., et al.
Dev. Brain Res., 79(2), 203-218 (1994)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service