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Sigma-Aldrich

Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Synonym(s):

Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.41

Quality Level

100

manufacturer/tradename

EZ-ChIP

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Components

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Quality

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A novel cis-acting element in Her2 promoter regulated by Stat3 in mammary cancer cells
Qian, Lu, et al
Biochemical and biophysical research communications null
Increased expression of hepatocyte nuclear factor 6 stimulates hepatocyte proliferation during mouse liver regeneration.
Yongjun Tan, Yuichi Yoshida, Douglas E Hughes, Robert H Costa
Gastroenterology null
Epigenetic regulation of the endothelial nitric oxide synthase gene in persistent pulmonary hypertension of the newborn rat.
Xu, XF; Ma, XL; Shen, Z; Wu, XL; Cheng, F; Du, LZ
Journal of Hypertension null
Seed-based systematic discovery of specific transcription factor target genes.
Mrowka, R; Bluthgen, N; Fahling, M
FEBS Journal null
D Pannell et al.
The EMBO journal, 19(21), 5884-5894 (2000-11-04)
Retrovirus vectors are de novo methylated and transcriptionally silent in mammalian stem cells. Here, we identify epigenetic modifications that mark retrovirus-silenced transgenes. We show that murine stem cell virus (MSCV) and human immunodeficiency virus type 1 (HIV-1) vectors dominantly silence
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